Calculation of Tm for PCR primers

conc (nM) Salt (mM)
Calculation method: Nearest Neighbor GC
Enter PCR primers:
forward:
reverse: 










 Tm GC mol%sequencelength A  T  G  C 



    forward
     mer



    reverse
     mer



    Diff.








    rev. compl. fwd.


	rev. compl. rev.